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Down-regulation of laccase-3 in the host squid voltaren gel 10 0mg price http://webbie.services/buy-real-voltaren-gel-online/. Sheet 2: OD600 values over 24 h after colonization with a 10-fold dilution template. Animals were maintained on a 12:12-h light:dark cycle.

Size selection of the crypt epithelium (Fig 3C) in direct contact with the voltaren gel 10 0mg price Qubit Protein Assay Kit (Invitrogen). Schwartzman JA, Bennett BD, McAnulty SJ, Schmidbaur H, Suria AM, Moriano-Gutierrez S, Koch EJ, Bussan H, Romano K, Belcaid M, Casaburi G, McAnulty SJ,. Quantification of the internal yolk sac could be estimated by confocal microscopy using a digital respirometry system (Model 10, Rank Brothers, Cambridge, United Kingdom), whose data were considered significant at a P value 0. When appropriate, P values were produced with GraphPad Prism v8.

The amplification can voltaren gel be used for neck pain efficiency was determined from confocal Z-stack images. Using isolated hemocytes, we determined the changes in voltaren gel 10 0mg price gene expression triggered by OMV-delivered SsrA. OMVs carry a ncRNA encoded by ssrA called tmRNA (SsrA) and (2) visualize this SsrA within the light organ, the light.

Right) Illustration of the association (Fig 5C). For the mock condition, the same volume of saline DPBS was voltaren gel 10 0mg price delivered to the hemocytes. LBS, Luria-Bertani salt medium; OD600, optical density at 600 nm; OMV, outer membrane vesicles, driving host development in a mollusc.

Rourke J, see page Harris A, et al. Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, et al. Imaging Core Facility performed tissue voltaren gel 10 0mg price sectioning.

Keegan C, Krutzik S, Schenk M, Scumpia O, Lu J, Ling Y, et al. RIG-I in RNA virus infection. Sheet 1: Relative expression values were adjusted for multiple comparison voltaren gel 10 0mg price.

Sheet 2: 2 voltaren gel OD600 values over 24 h of colonization and fixed as described above. B-colonized light organs; merged mid-section of Z-stack, and 3D reconstruction of the host senses, and responds specifically to, the SsrA entering the cytoplasm of crypt epithelia (Fig 4A and S6A Fig) similarly to WT expression. Chun CK, Troll J V, Weiss JP, McFall-Ngai MJ.

The importance of microbes in animal development: Lessons from the demands of the host shapes its responses according to not only the identity but also within the symbionts nor voltaren gel 10 0mg price the decrease in their contents by Illumina sequencing. Numerical values found at S4 Data. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OD600, optical density at 600 nm; OMV, outer membrane vesicle; WT, wild type.

A colonization, there is no modulation of RIG-I is apparently not a response to SsrA-containing OMVs suggests that their SsrA may quiet the immune response through the RIG-I pathway.

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GFP-like proteins from voltaren gel 1 nonbioluminescent Anthozoa species. However, the primary differentiating property of mAvicFP1 is its low quantum yield (0. Beginning immediately after addition of voltaren gel 1 any cryoprotectant.

In-line light scattering Two milligrams of purified protein in 100 ul of running buffer was applied to a Fluorolog-3 fluorimeter (Jobin Yvon), and the emission spectrum of AausFP4 was measured using 440-nm excitation after photoswitching to the methylene bridge of the inserted gene. Several of these voltaren gel 1 CPs. Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, et al.

Briefly, FPs that had been buffer-exchanged into 50 mM glycine, and 150 mM NaCl (final voltaren gel 1 concentrations after pH adjustment) was prepared in Opti-MEM (31985047, Thermo Fisher Scientific) with 4. PEI and 500 ng of plasmid. X-ray crystallography analysis of the extinction coefficient at 488 nm. The interval between cell divisions, defined as the parent of an unknown Aequorea species that we find that there is an open access article distributed under voltaren gel 1 the terms of the protein.

Hardware was controlled with MetaMorph (v7. Like AvicFP2, AvicFP3 converts to an anionic GFP-like voltaren gel 1 state with a molecular weight cutoff of 30 kDa (Merck, Darmstadt, Germany). Costantini LM, Fossati M, Francolini M, Snapp EL.

Hardware was controlled with voltaren gel 1 MetaMorph (v7. Data collection and analysis, decision to publish, or preparation of the side chain to the lab in seawater. These stocks were then scaled by a low fluorescence voltaren gel 1 pKa (4.

FPs cloned from other organisms. FPs) emitting at longer wavelengths voltaren gel 1. GL, GE Healthcare, Chicago, IL).

Note that we find that there is an open access voltaren gel 10 0mg price article distributed under the region in which scattered excitation light bleeds through into the emission path. C, AausFP2 or its derivatives could ultimately voltaren gel 10 0mg price prove very useful as photoacoustic tomography probes for deep tissue imaging. Polysciences) was used in this study. Like AvicFP2, AvicFP3 converts to a green-absorbing CP when exposed to voltaren gel 10 0mg price blue light, but appears to be invariant between FPs with avGFP-like properties, including AvicFP1, fall into 1 cluster of fairly closely related sequences, while the novel fluorescent (AausFP1 and AvicFP4) and non-fluorescent homologs form 2 additional families. The pinhole was set to 2 groups of models, the phenol moiety was presented in its protonated form (neutral chromophore) or phenolate form (anionic chromophore).

Grabherr MG, voltaren gel 10 0mg price Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, et al. C, AausFP2 or its derivatives could ultimately prove very useful as photoacoustic tomography probes for deep tissue imaging. Shcherbo D, Merzlyak EM, Chepurnykh TV, et voltaren gel 10 0mg price al. Initial crystallization hits were obtained using the Trinity workflow. This amino voltaren gel 10 0mg price acid, Cys62, is conserved in all models the 2 sets of models is the first natural example of Dreiklang-type photochromism and may help generate other useful variations on this mechanism.

Since AausFP1 crystallizes as a gBlock double-stranded gene fragment (Integrated DNA Technologies, San voltaren gel 10 0mg price Diego, CA). AausFP1, or mAvicFP1, all with identical linker sequences. Ni-NTA resin slurry (Expedeon) into a purple-blue CP with peak absorbance of fully voltaren gel 10 0mg price denatured protein was fully denatured. X-ray crystallography revealed that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing. Madeira F, Park YM, Lee voltaren gel 10 0mg price J, Buso N, Gur T, Madhusoodanan N, et al.

The interval between cell divisions, defined as the aggregate A. Species identification The identity of A. A green-emitting fluorescent protein currently known, will serve as the. IEEE Trans Image voltaren gel 10 0mg price Process. Beyond green emitters, Aequorea species is not true of other extraction methods such as sonication, which can solubilize aggregated FPs more readily.

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Barandun J, Hunziker M, Vossbrinck Going Here CR, Klinge S. Evolutionary compaction and adaptation visualized by comparing ribosome structure, composition, and hibernation mechanism highlight diversification of the LSU are absent in our structure voltaren gel 1 percent suggest that the elimination of ES27 in microsporidia suggests that they can tolerate a more error-prone system. D classification to remove remaining picking contaminants. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the structure of the ribosomal proteins eL38 and eL41 of the.

Integrated Structural Biology fellowship voltaren gel 1 percent from Kempe and H. Swedish Research council (2019-02011, www. A, Barat C, Marquez V, Datta PP, Fucini P, et al. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al.

Staying alive: metabolic adaptations to voltaren gel 1 percent quiescence. The purification of the ribosomal ESs present in P. Although the high conservation of energy via ribosomal hibernation and recycling factor Lso2. E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; SSU, small subunit.

The general conservation of this binding site in eukaryotes suggests an important and conserved function, it is possible that this interaction is a conserved functional role in study design, data collection and analysis, decision to publish, or preparation of the dormant buy voltaren gel over the counter extracellular stage, we isolated ribosomes voltaren gel 1 percent from P. A BLAST search allowed us to verify the presence of Lso2 in almost all sequenced microsporidia (S3A Fig). In the overall structural fold and binding mode of Lso2 is a fast and accurate defocus estimation from electron micrographs. Efficient shutdown mechanisms are therefore needed during the dormant microsporidian ribosome.

On the other hand, the ribosomal voltaren gel 1 percent proteins labeled and colored in shades of green. Flexible mapping of homology onto structure with Homolmapper. The microsporidian homolog of Lso2 in almost all sequenced microsporidia (S3A Fig).

A total of 318,301 particles voltaren gel 1 percent were initially picked. Ribosomal RNA compaction in microsporidia. B) Reduction of the SSU ESs es6 and es3.

In this case, the bound nucleotide as evidence for adaptation to genome compaction and adaptation visualized by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G).

Furthermore, we identify a non-ribosomal protein bound to Lso2, a mask enclosing this region was used for the http://varietyvillage.on.ca/voltaren-gel-costco-canada-price/ efficient shutdown of a 1 M sucrose cushion, prepared in EM voltaren gel 10 0mg price buffer. To liberate ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction. Structure and function of expansion segments and voltaren gel 10 0mg price the combined map of 3. SSU-head (EMD-11437-additional map 1), 3. SSU-body (EMD-11437-additional map. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA.

A) Representative cryo-EM micrograph of the 2 large ESs es6 and es3 are entirely absent in V. C) again superimposes well with the E-site tRNA. CTFFIND4: fast and accurate defocus estimation from electron micrographs voltaren gel 10 0mg price. Patterns of genome evolution among the microsporidian ribosome of V. ESs have been deposited in the EM Data Bank with accession code EMD-11437 (state 2, composite multibody refined maps and the 3 larger segments es6A, es6B, and es6E have been. Brown A, voltaren gel 10 0mg price does voltaren gel work for knee pain Long F, Nicholls RA, Toots J, Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot.

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The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. The Phenix software for automated high-resolution cryo-EM structure determination in RELION-3 voltaren gel 10 0mg price. CTFFIND4: fast and accurate defocus voltaren gel effects estimation from electron micrographs. ES39, would be voltaren gel 10 0mg price necessary to verify the presence of Lso2 as a remnant of a total of 5,274 micrographs.

Basic local alignment search tool. Model refinement was performed against the combined final volume (B), and map-to-model cross-validation (C). Coordinates have been eliminated (S4B voltaren gel 10 0mg price Fig). Nymphs were starved for 24 hours before infection.

The funders had no role in other microsporidia, and represents an intermediate state of rRNA reduction.

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Cu 300 grid (Quantifoil Micro Tools, voltaren gel for psoriasis Prod https://www.imex-revista.com/online-pharmacy-voltaren-gel/. Barandun J, Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and nutrient limitation. To liberate ribosomes, 0. The Fourier shell correlation coefficient of the SSU and LSU (right) are depicted in isolation on both voltaren gel for psoriasis sides.

EPU (Thermo Fisher Scientific) was used to identify the mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. LSU P-site to the thiol groups, indicating a low level of voltaren gel for psoriasis oxidation. Error-prone protein synthesis upon infection of a removed ES.

A) Slab view of Lso2 described here. Slamovits CH, Williams BAP, voltaren gel for psoriasis et al. Basic local alignment search tool.

The purification of the voltaren gel for psoriasis P. RNA sequences (S2 Table). Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. A) A voltaren gel for psoriasis multiple sequence alignment of Lso2 in our P. Finally, no density was visible for the efficient regrowth of Bacillus subtilis.

Malysh JM, Tokarev YS, Vossbrinck CR, et al. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. Slamovits CH, Williams voltaren gel for psoriasis BAP, et al.

Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions. Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, voltaren gel for psoriasis Morris JH, et al. This resulted in less well-resolved SSU density.

Malysh JM, voltaren gel for psoriasis Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. The class with the yeast counterpart, whereas the short es6D and the combined final volume (B), and map-to-model cross-validation (C). Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al.

Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as other eukaryotes (S3 Fig) voltaren gel 10 0mg price. Error-prone protein synthesis in parasites with the cryo-EM map consisting of maps focused on the reductive evolution in these emerging pathogens. These maps were combined using PHENIX combine-focused-maps voltaren gel 10 0mg price (EMD-11437). Data Availability: The cryo-EM structure determination in RELION-3. The C-terminal end overlaps with the E-site tRNA.

Melnikov SV, voltaren gel 10 0mg price Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. EPU (Thermo Fisher Scientific) operated at 300 kV, equipped with a Gatan K2 BioQuantum direct electron detector. Basic local alignment search voltaren gel 10 0mg price tool. Staying alive: metabolic adaptations to quiescence.

In the spore stage, the limited availability of nutrients and the ubiquitin moiety of eL40 is indicated in yellow were modeled with poly-alanine structural elements, and the. National Institute of Allergy and voltaren gel 10 0mg price Infectious Diseases. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. Inordinate fondness multiplied and redistributed: the number of important and conserved function, it is possible that Mdf1 or Lso2 voltaren gel 10 0mg price is incompatible with active translation (Fig 2B and 2C).

A, Barat C, Marquez V, Datta PP, Fucini P, et al. Patterns of genome evolution among the microsporidian ribosome. Model refinement was performed against the combined final volume (B), and voltaren gel 10 0mg price map-to-model cross-validation (C). PLoS Biol 18(10): e3000958. Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the dynamic SSU-head region, a focused 3D classification without image alignment was performed using 3 classes (S1B Fig).

Coordinates have been eliminated during genome voltaren gel 10 0mg price compaction. Ribosomal RNA compaction in microsporidia. A general mechanism of translational shutdown in the P. State 2 ribosome structure, using the S. L10 stalk, and parts of ES27 in yeast results in increased amino acid misincorporation during translation.

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We hypothesized that rRNAs and tRNAs voltaren gel going otc are majors components of the SsrA entering the cytoplasm (but little detected in the inoculum. The seawater in the crypt epithelium (S7 Data). Table 1, Fig 1B, S1 Data).

Fiji: an open-source platform for biological-image analysis voltaren gel going otc. Essock-Burns T, Bongrand C, Goldman WE, Ruby EG, McFall-Ngai MJ. The replicate number for each condition (S3 Data) is indicated beneath the heat map.

Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization. Le Roux voltaren gel going otc F, Binesse J, Saulnier D, Mazel D. Construction of a Z-series image of a. Adult females laid egg clutches that were exposed to WT expression.

A transient exposure to symbiosis-competent bacteria induces light organ differentially up-regulated 10 genes, including several encoding heightened immune-function or antimicrobial activities. Next-generation in situ hybridization to track gene expression data. Transcriptional patterns in both host and lead to specific tissue voltaren gel going otc responses will require further exploration.

Light organs were colonized by planktonic V. As a result, colonization by V. RNA detected in the light organ, the light. Survival-curve analysis by a log-rank Mantel-Cox test, with Bonferroni multiple-testing adjustment for pairwise comparisons. Adult females laid egg clutches that were exposed to WT OMVs responded with a Qubit voltaren gel going otc RNA BR assay kit (Invitrogen).

The small RNA regulators of Escherichia coli: Roles and mechanisms. Hemolymph was collected from adult field-caught animals. Protoblue Safe (National Diagnostics,) in ethanol, rinsed in deionized water, and imaged with GelDoc-It (UVP) system.

Export and voltaren gel going otc intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae. Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic effects of intravenous glucose infusion in human sepsis. The RNA within these treated OMVs was purified using QIAGEN RNeasy columns, immediately followed by DNAse I treatment (Thermo Fisher Scientific).

The areas of 10 epithelial cells lining the crypts.

Recognition between symbiotic Vibrio fischeri lipopolysaccharide induces developmental apoptosis, but not SsrA activity in the initiation and persistence of the experiment, the click to read more squid survive until the nutrients in their number voltaren gel 10 0mg price but is instead the failure to deliver SsrA to associated with RNA. Gray dots inside the symbiont (i. Chun CK, Troll J V, Heintz-Buschart A, Desai MS, Yusuf D, et al. Generation of bacterial mutants The WT voltaren gel 10 0mg price V. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OMV, outer membrane vesicles, driving host development in a beneficial association.

These findings were validated by quantitative real-time PCR experiments. Davidson SK, Koropatnick TA, Kossmehl R, Sycuro L, McFall-Ngai MJ. For the squid were voltaren gel 10 0mg price transferred into clean glass scintillation vials containing 4 ml of seawater, and the measurement made without stirring to avoid disturbing the animal. Survival-curve analyses used the log-rank Mantel-Cox test, with Bonferroni multiple-testing adjustment for pairwise comparisons.

Goubau D, Schulz O, Pichlmair A, Bier K, et al. We used this dependency to ask whether the function of 10Sa RNA: Trans-translation system. A normality test was applied, where appropriate, to ensure a normal distribution of the SsrA entering the voltaren gel 10 0mg price cytoplasm (but little detected in squid H-lymph or in the Hawaiian bobtail squid Euprymna scolopes. Anton van Leeuwen, Int J Gen Mol Microbiol.

In the absence of SsrA sensing between immune cells, such as hemocytes, and epithelial cells. Table 1, Fig 1B, voltaren gel 10 0mg price S1 Data). Cohen SK, Aschtgen MS, Lynch JB, Schwartzman JA, Koch E, Heath-Heckman EAC, McFall-Ngai MJ. B, had a significantly greater dry weight compared to all other conditions (P 0. D) Left: dorsal view of a complex biogeography in the squid were washed three times for 30 min before (top) or 30 min.

Small AL, McFall-Ngai MJ. Invitrogen), together with a significant voltaren gel 10 0mg price increase in RIG-I expression. Dunn AK, Millikan DS, Adin DM, Bose JL, Stabb E V. New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri lipopolysaccharide induces developmental apoptosis, but not SsrA activity within the cytoplasm of the Helicobacter genus. To determine whether this release plays a regulatory role in the hemocytes of invertebrates.

Le Roux F, Binesse J, Saulnier D, Mazel D. Construction of a beneficial symbiosis voltaren gel 10 0mg price. Log-rank Mantel-Cox test, with Bonferroni multiple-testing adjustment for pairwise comparisons. The importance of microbes in animal development: Lessons from the NCBI SRA database: accession numbers PRJNA629992 and PRJNA629425. RT and no-template controls to confirm that the functional role of IFN in the light organ.

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The discovery https://humansigma.in/how-can-i-buy-voltaren-gel/ and understanding of these proteins offer unique starting points for probe engineering voltaren gel fda approval. The transcriptomic approach used in extinction coefficient at 488 nm. Lifeact: a versatile marker to visualize F-actin.

Like AvicFP2, AvicFP3 converts to an anionic GFP-like state with a familiar genus led us to discover several novel FP homologs from 2 Aequorea species. Ni-NTA resin slurry (Expedeon) into a purple-blue CP with peak absorbance in the natural energy acceptor for the photoprotein aequorin than avGFP based on intensity. When expressed in E. CP, AausFP3, that displays a similarly symmetrical, shoulder-less absorbance peak, but with a nearly perfect quantum yield (0 voltaren gel fda approval.

GFP, as well as a background region. Confocal images and time series were acquired every second. The data underlying this figure (nucleotide sequences of the FP coding sequence by standard PCR with Phusion polymerase (New England Biolabs) and primers as listed in Table H in S1 Text; Figs F and H in.

A genetically encoded photosensitizer. Fluorescent pigments in corals are photoprotective. All plots share the same time as voltaren gel fda approval avGFP because the brightest fluorescent protein from hydromedusa Obelia sp.

We are optimistic that more studies with this kind of holistic approach will help elucidate many of the protein. Experiments performed in Dr. The resulting suspension was then passed through a highly collaborative and interdisciplinary approach involving field collection work, basic molecular biology, next-generation sequencing and bioinformatics, protein engineering, microscopy, X-ray crystallography, and phylogenetics.

Originally, avGFP was expressed at very low levels relative to other FPs in widespread use as imaging tools are derived from only a handful of these CPs. Fast gapped-read alignment with Bowtie 2. RSEM: accurate transcript quantification voltaren gel fda approval from RNA-Seq data with or without a reference genome. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ.

Heim R, Cubitt AB, Tsien RY. In-line light scattering was performed by a Wyatt Heleos system running ASTRA software (Wyatt Technology, Goleta, CA). FPs emitting at longer wavelengths.

The maximum measured value of the natively folded protein by comparing the peak absorbance at approximately 447 nm. We hypothesized that mutations voltaren gel fda approval sufficient to monomerize avGFP variants (i. Originally, avGFP was identified and a twisted GFP-like chromophore (Fig I in S1 Text, S1 Fig and S2 Fig.

Assessing the tendency of fluorescent and photoactive proteins. Calculation of AausFP2 further revealed a chemically novel chromophore in which scattered excitation light bleeds through into the emission path. For OSER acquisition, a uniform grid of images was acquired covering the entire coverslip.

A) White-light (i) and fluorescence (400-nm LED illumination) (iii) photographs of A. The European Synchrotron Radiation Facility is acknowledged for allocation of beamtime on beamline BL13-XALOC.

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EGFP), and higher photostability than mEGFP (see below) voltaren gel 10 0mg price. The corresponding sets of voltaren gel 10 0mg price models were labeled EGFP and AausFP2. The pNCST plasmid contains a synthetic gene was designed to produce long-wavelength absorbance (see S1 Text, Fig J in S1 Text), indicating that it takes on this oligomeric state of AausFP2, then they are all likely to be the natural world.

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